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deepvent exo dna polymerase  (New England Biolabs)


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    Structured Review

    New England Biolabs deepvent exo dna polymerase
    Deepvent Exo Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deepvent+exo+dna+polymerase/Deep+Vent+(exo/us12553044-608-22-25
    Average 95 stars, based on 334 article reviews
    deepvent exo dna polymerase - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Lysis:

    Article Title: An integrated view of the structure and function of the human 4D nucleome.
    Article Snippet: .. The lysis buffer containing the extracted genomic DNA was mixed with 2× DeepVent mix buffer (2× Thermo polymerase buffer (10×), 400 μm dNTPs, 4 mM MgSO4 in ultrapure DNase-free water), 0.5 μM GAT-7N primers (5′-GTGAGTGATGGTTGAGGTAGTGTGGAGNNN NNNN) and 2 U μl−1 DeepVent (exo-) DNA polymerase (New England Biolabs, M0259L) and incubated for 11 cycles in the BioRad thermocycler. ..

    Article Title: Extensive folding variability between homologous chromosomes in mammalian cells
    Article Snippet: .. The lysis buffer containing the extracted genomic DNA was mixed with 2× DeepVent mix buffer (2× Thermo polymerase buffer (10x), 400 μm dNTPs, 4 mM MgSO 4 in ultrapure DNase free water), 0.5 μM GAT-7N primers (5′-GTG AGT GAT GGT TGA GGT AGT GTG GAG NNN NNN N) and 2 units/μl DeepVent ® (exo-) DNA polymerase (New England Biolabs, M0259L), and incubated for 11 cycles in the BioRad thermocycler. ..

    Article Title: An integrated view of the structure and function of the human 4D nucleome
    Article Snippet: .. The lysis buffer containing the extracted genomic DNA was mixed with 2× DeepVent mix buffer (2× Thermo polymerase buffer (10×), 400 μm dNTPs, 4 mM MgSO 4 in ultrapure DNase-free water), 0.5 μM GAT-7N primers (5′-GTGAGTGATGGTTGAGGTAGTGTGGAGNNNNNNN) and 2 U μl −1 DeepVent (exo-) DNA polymerase (New England Biolabs, M0259L) and incubated for 11 cycles in the BioRad thermocycler. ..

    Article Title: Extensive folding variability between homologous chromosomes in mammalian cells.
    Article Snippet: .. The lysis buffer containing the extracted genomic DNA was mixed with 2× DeepVent mix buffer (2× Thermo polymerase buffer (10x), 400 μm dNTPs, 4 mM MgSO4 in ultrapure DNase free water), 0.5 μM GAT7N primers (5′-GTG AGT GAT GGT TGA GGT AGT GTG GAG NNN NNN N) and 2 units/μl DeepVent® (exo-) DNA polymerase (New England Biolabs, M0259L), and incubated for 11 cycles in the BioRad thermocycler. ..

    Incubation:

    Article Title: An integrated view of the structure and function of the human 4D nucleome.
    Article Snippet: .. The lysis buffer containing the extracted genomic DNA was mixed with 2× DeepVent mix buffer (2× Thermo polymerase buffer (10×), 400 μm dNTPs, 4 mM MgSO4 in ultrapure DNase-free water), 0.5 μM GAT-7N primers (5′-GTGAGTGATGGTTGAGGTAGTGTGGAGNNN NNNN) and 2 U μl−1 DeepVent (exo-) DNA polymerase (New England Biolabs, M0259L) and incubated for 11 cycles in the BioRad thermocycler. ..

    Article Title: Extensive folding variability between homologous chromosomes in mammalian cells
    Article Snippet: .. The lysis buffer containing the extracted genomic DNA was mixed with 2× DeepVent mix buffer (2× Thermo polymerase buffer (10x), 400 μm dNTPs, 4 mM MgSO 4 in ultrapure DNase free water), 0.5 μM GAT-7N primers (5′-GTG AGT GAT GGT TGA GGT AGT GTG GAG NNN NNN N) and 2 units/μl DeepVent ® (exo-) DNA polymerase (New England Biolabs, M0259L), and incubated for 11 cycles in the BioRad thermocycler. ..

    Article Title: An integrated view of the structure and function of the human 4D nucleome
    Article Snippet: .. The lysis buffer containing the extracted genomic DNA was mixed with 2× DeepVent mix buffer (2× Thermo polymerase buffer (10×), 400 μm dNTPs, 4 mM MgSO 4 in ultrapure DNase-free water), 0.5 μM GAT-7N primers (5′-GTGAGTGATGGTTGAGGTAGTGTGGAGNNNNNNN) and 2 U μl −1 DeepVent (exo-) DNA polymerase (New England Biolabs, M0259L) and incubated for 11 cycles in the BioRad thermocycler. ..

    Article Title: Extensive folding variability between homologous chromosomes in mammalian cells.
    Article Snippet: .. The lysis buffer containing the extracted genomic DNA was mixed with 2× DeepVent mix buffer (2× Thermo polymerase buffer (10x), 400 μm dNTPs, 4 mM MgSO4 in ultrapure DNase free water), 0.5 μM GAT7N primers (5′-GTG AGT GAT GGT TGA GGT AGT GTG GAG NNN NNN N) and 2 units/μl DeepVent® (exo-) DNA polymerase (New England Biolabs, M0259L), and incubated for 11 cycles in the BioRad thermocycler. ..

    Random Hexamer:

    Article Title: A single dose of cocaine rewires the 3D genome structure of midbrain dopamine neurons
    Article Snippet: Briefly, NPs were lysed for 4 or 24 h at 60°C in lysis buffer (final concentration: 30 mM Tris-HCl pH 8.0, 2 mM EDTA pH 8.0, 800 mM Guanidinium-HCl, 5 % (v/v) Tween 20, 0.5 % (v/v) Triton X-100) and 2.116 units/ml QIAGEN protease (Qiagen, 19155), before a 30 min protease inactivation at 75°C. .. Pre-amplification was done using a 2x DeepVent mix (2x Thermo polymerase buffer (10x), 400 μM dNTPs, 4 mM MgSO 4 in ultrapure water), 0.5 μM GAT-7N random hexamer primers with an adapter sequence and 2 units/μl DeepVent ® (exo-) DNA polymerase (New England Biolabs, M0259L). ..

    Sequencing:

    Article Title: A single dose of cocaine rewires the 3D genome structure of midbrain dopamine neurons
    Article Snippet: Briefly, NPs were lysed for 4 or 24 h at 60°C in lysis buffer (final concentration: 30 mM Tris-HCl pH 8.0, 2 mM EDTA pH 8.0, 800 mM Guanidinium-HCl, 5 % (v/v) Tween 20, 0.5 % (v/v) Triton X-100) and 2.116 units/ml QIAGEN protease (Qiagen, 19155), before a 30 min protease inactivation at 75°C. .. Pre-amplification was done using a 2x DeepVent mix (2x Thermo polymerase buffer (10x), 400 μM dNTPs, 4 mM MgSO 4 in ultrapure water), 0.5 μM GAT-7N random hexamer primers with an adapter sequence and 2 units/μl DeepVent ® (exo-) DNA polymerase (New England Biolabs, M0259L). ..

    Polymerase Chain Reaction:

    Article Title: Methylation detection and analysis of mammalian DNA
    Article Snippet: .. After transposition and transposase removal, a PCR reaction mixture including Mg2+, dNTP mix, primers and a thermal stable DNA polymerase such as Deepvent exo-DNA polymerase (New England Biolabs) is added to the solution at a suitable temperature and for a suitable time period to fill the 9 bp gap left by the transposition reaction. ..



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    New England Biolabs deepvent exo polymerases
    (a)PCR of a five-symbol data storage gene assembly with different <t>polymerases</t> with and without ET SSB. Expected gene length is 583bp. (b) PCR amplification of three data storage genes assembled with DNAzymes with <t>DeepVent</t> (exo-) polymerase.
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    Image Search Results


    (a)PCR of a five-symbol data storage gene assembly with different polymerases with and without ET SSB. Expected gene length is 583bp. (b) PCR amplification of three data storage genes assembled with DNAzymes with DeepVent (exo-) polymerase.

    Journal: bioRxiv

    Article Title: Directed assembly of single-stranded DNA fragments for data storage via enzyme-free catalytic splint ligation

    doi: 10.1101/2024.10.09.617455

    Figure Lengend Snippet: (a)PCR of a five-symbol data storage gene assembly with different polymerases with and without ET SSB. Expected gene length is 583bp. (b) PCR amplification of three data storage genes assembled with DNAzymes with DeepVent (exo-) polymerase.

    Article Snippet: DeepVent and DeepVent (exo-) polymerases and PCR buffers were purchased from NEB.

    Techniques: Amplification